
Science of NAD
How are your NAD levels today?


Feb 6, 2023
Metabolism - Heart / Vascular - NMN - Obesity
Journal of Clinical Endocrinology & Metabolism
Nicotinamide Adenine Dinucleotide Augmentation in Overweight or Obese Middle-Aged and Older Adults: A Physiologic Study
Pencina, Karol Mateusz
Feb 4, 2023
Heart / Vascular - Aging
Antioxidants
NAD+ Homeostasis and NAD+-Consuming Enzymes: Implications for Vascular Health
Campagna, Roberto
SUMMARY
Although future studies are required to better clarify the potential of targeting NAD+ homeostasis, the strategies based on administering NAD+ precursors or inhibiting NAD+-dependent enzymes seem to be an attractive approach for reducing chronic low-grade inflammation, enhancing mitochondrial biogenesis, and improving oxidative metabolism in vascular cells.
Dec 31, 2022
Kidney - Heart / Vascular
Journal of Molecular and Cellular Cardiology
Nicotinamide riboside improves MI-induced acute kidney injury through increasing nicotinamide phosphorybosil transferase and NAD renal levels
Habeichi, Nada
Oct 11, 2022
Circadian Rhythm - Aging - Heart
American Journal of Physiology - Cell Physiology
Circadian cardiac NAD+ metabolism, from transcriptional regulation to healthy aging
Carpenter, Bryce J.
SUMMARY
...Recent studies have expanded on the role of circadian rhythms and the core clock factors that maintain them in the regulation of NAD+ levels in the heart. This has revealed that NAD+ pools and their use are tightly linked to cardiac function, but also heart failure. The convergence of these fields, namely, clock regulation, heart disease, and NAD+ metabolism present a complex network ripe with potential scientific and clinical discoveries, given the growing number of animal models, recently developed technology, and opportunity for safe and accessible precursor supplementation...
Oct 1, 2022
Heart / Vascular - Mitochondrial Disorder
Molecular Metabolism
Nicotinamide riboside alleviates exercise intolerance in ANT1-deficient mice
Schaefer, Patrick
Sep 14, 2022
Heart / Vascular - Aging
Journal of Cardiovascular Aging
Metabolic targets in cardiac aging and rejuvenation
Liu, Chang
SUMMARY
NAD+ repletion can delay several hallmarks of aging and suppress the deterioration of age-related diseases[95]. This suggests a significant potential for the treatment of cardiac diseases in the elderly population with supplementation of NAD+ precursors. Indeed, the dietary intake of NAM could reduce cardiac hypertrophy and diastolic dysfunction in aged mice
Sep 14, 2022
Heart / Vascular
JACC: Basic to Translational Science
Safety and Tolerability of Nicotinamide Riboside in Heart Failure With Reduced Ejection Fraction
O'Brien, Kevin
SUMMARY
The mitochondrial dysfunction characteristic of heart failure (HF) is associated with changes in intracellular nicotinamide adenine dinucleotide (NAD+) and NADH levels. Raising NAD+ levels with the NAD+ precursor, nicotinamide riboside (NR), may represent a novel HF treatment. In this 30-participant trial of patients with clinically stable HF with reduced ejection fraction, NR, at a dose of 1,000 mg twice daily, appeared to be safe and well tolerated, and approximately doubled whole blood NAD+ levels...
May 9, 2022
Heart - Vascular - Circadian Rhythm - Aging
Frontiers in Molecular Medicine
Nicotinamide Riboside Improves Cardiac Function and Prolongs Survival After Disruption of the Cardiomyocyte Clock
Dierickx, Pieterjan
SUMMARY
Here, we show that supplementation with the NAD+ precursor NR as a dietary supplement improves heart function and extends the lifespan of female mice lacking cardiac REV-ERBs. Thus, boosting NAD+ levels can improve cardiac function in a setting of heart failure caused by disruption of circadian clock factors, providing new insights into the links between the circadian clock, energy metabolism, and cardiac function.
Dec 3, 2021
Heart / Vascular
Antioxidants
Therapeutic Potential of Emerging NAD+-Increasing Strategies for Cardiovascular Diseases
Rotllan, Noemi
SUMMARY
Compelling evidence suggests that NAD+ homeostasis is required for normal cardiovascular function. Numerous studies suggest that declining NAD+ concomitant with mitochondrial dysfunction is a common trait in myocardial and cardiovascular diseases...Except for NA, which causes some adverse clinical outcomes, vitamin B3 intermediates have been demonstrated to be safe and to have promising beneficial effects in combating cardiovascular diseases in vivo...
Nov 29, 2021
Reproduction - Pregnancy - Stem Cells - Heart / Vascular
Cell Death & Disease
Nicotinamide promotes cardiomyocyte derivation and survival through kinase inhibition in human pluripotent stem cells
Meng, Ya
Aug 27, 2021
Heart / Vascular
Frontiers in Cardiovascular Medicine
Enhancing NAD+ Metabolome in Cardiovascular Diseases: Promises and Considerations
Jahan, Fahmida
Apr 22, 2021
Heart / Vascular
Circulation Research
NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction
Tong, Dan
SUMMARY
...We tested whether oral NR could correct the NAD+ deficiency and improve mitochondrial function in Heart failure with preserved ejection fraction (HFpEF). Remarkably, after full development of HFpEF (5 weeks of HFD+L-NAME), 8 weeks of NR supplementation ameliorated NAD+ deficiency and reversed hyperacetylation of VLCAD in animals maintained on HFpEF treatment...We have discovered that oral NR effectively corrected cardiac NAD+ deficiency, reversed the hyperacetylation status of key FAO enzymes, and improved mitochondrial function in our murine HFpEF model. This correlated with remarkable attenuation of left ventricular hypertrophy and diastolic dysfunction and dramatic improvement of HFpEF functional manifestations, suggesting that NR could potentially emerge as a promising therapeutic agent for HFpEF. It is worth emphasizing that restoration of all these parameters occurred in the continued presence of the HFpEF-triggering comorbidities of mechanical and metabolic stress.
Apr 1, 2021
Heart / Vascular
Clinica Chimica Acta
NAD+ and cardiovascular diseases
Lin, Qiuzhen
SUMMARY
...NAD+ protects against metabolic syndrome, heart failure, ischemia-reperfusion (IR) injury, arrhythmia and hypertension. Accordingly, the subsequent loss of NAD+ in aging or during stress results in altered metabolic status and potentially increased disease susceptibility. Therefore, it is essential to maintain NAD+ or reduce loss in the heart. This review focuses on the involvement of NAD+ in the pathogenesis of cardiovascular disease and explores the effects of NAD+ boosting strategies in cardiovascular health.
Dec 15, 2020
Heart / Vascular - Obesity
Life Sciences
Nicotinamide riboside reduces cardiometabolic risk factors and modulates cardiac oxidative stress in obese Wistar rats under caloric restriction
Macedo de Castro, Josimar
SUMMARY
This is the first study to report effects of nicotinamide riboside on cardiac oxidative stress in an obesity model. Nicotinamide riboside, a natural dietary compound, presented antiobesity effects and cardiometabolic benefits, in addition to positively modulating oxidative stress in the heart, in a state-dependent manner.
Oct 12, 2020
Heart / Vascular - Inflammation
Journal of Clinical Investigation
Boosting NAD level suppresses inflammatory activation of PBMCs in heart failure
Zhou, Bo
SUMMARY
While mitochondria play an important role in innate immunity, the relationship between mitochondrial dysfunction and inflammation in heart failure is poorly understood. In this study we aimed to investigate the mechanistic link between mitochondrial dysfunction and inflammatory activation in peripheral blood mononuclear cells (PBMCs), and the potential antiinflammatory effect of boosting the NAD level. ...Oral nicotinamide riboside (NR) administration enhanced PBMC respiration and reduced proinflammatory cytokine gene expression in 4 subjects with heart failure. These findings suggest that systemic inflammation in patients with heart failure is causally linked to mitochondrial function of the PBMCs. Increasing NAD levels may have the potential to improve mitochondrial respiration and attenuate proinflammatory activation of PBMCs in heart failure.
Aug 1, 2020
Heart / Vascular
Biochemical Pharmacology
Reversal of endothelial dysfunction by nicotinamide mononucleotide via extracellular conversion to nicotinamide riboside
Mateuszuk, Lukasz
SUMMARY
NMN and NR modulate intracellular NAD content in endothelium, inhibit endothelial inflammation and improve NO-dependent function by CD73-dependent and independent pathways, respectively. Extracellular conversion of NMN to NR by CD73 localized in the luminal surface of endothelial cells represent important vasoprotective mechanisms to maintain intracellular NAD.
Mar 21, 2020
Heart / Vascular
Journal of Molecular and Cellular Cardiology
Modulation of the Cardiac Sodium Channel Nav1.5 Peak and Late Currents by NAD+ Precursors
Matasic, Daniel S.
Jan 14, 2020
Heart / Vascular
Neuroscience and Therapeutics
Nicotinamide riboside rescues angiotensin II–induced cerebral small vessel disease in mice
Li, Cheng-Cheng
SUMMARY
Hypertension is a leading cause of cerebral small vessel disease (CSVD). Currently, treatments for CSVD are limited. Nicotinamide riboside (NR) can protect against vascular injury and cognitive impairment in neurodegenerative diseases. In this study, the protective effects of NR against angiotensin - (Ang -)–induced CSVD were evaluated...Our results indicated that NR administration alleviated Ang -–induced CSVD by protecting BBB integrity, vascular remodeling, neuroinflammation, and white matter injury (WMI)–associated cognitive impairment.
Jan 1, 2020
Heart / Vascular
Master's Thesis
The Effects of Nicotinamide Riboside on Postprandial Oxidative Stress and Vascular Function
Schiff, Isaac
Dec 1, 2019
Heart / Vascular
International Journal of Biochemistry and Cell Biology
NAD+ repletion inhibits the endothelial-to-mesenchymal transition induced by TGF-beta in endothelial cells through improving mitochondrial unfolded protein response
Zhang, Minxue
SUMMARY
Endothelial-to-mesenchymal transition (EndMT) plays an important role in the progression of cardiac fibrosis but its mechanism and treatment need to be further understood. Herein, we have found that...NAD + precursor nicotinamide riboside (NR) could attenuate TGF-β1-induced EndMT and improve the levels of mtUPR. Significantly, prohibitin proteins (PHB and PHB2) was also regulated by nicotinamide riboside...In conclusion, our results indicate that nicotinamide riboside improved the expression of prohibitin proteins to ameliorate EndMT via promotion of mtUPR. Nicotinamide riboside is a potential therapeutic target for cardiac fibrosis.